Lipid Extraction from Mouse Feces

Simultaneous determination mouse feces

A method fbr high-perfbrmance liquid chromatography (HPLC) has been estahlished fbr the simultaneous determination of rheinanthrone, sennidin A, scnnidin B and rhein formed by incubation f sennoside A with rat and mouse feces. The analysis was done within 20 min with a Wakosil-II5C18HG column by linear gradient elution using a mobile phase containing aqucous phosphoric acid and acetonitrile. Rh...

Lipid Extraction from Wheat Flour Using Supercritical Fluid Extraction

Cereal Chem. 81(6):693–698 Environmental concerns, the disposal cost of hazardous waste, and the time required for extraction in current methods encouraged us to develop an alternate method for analysis of wheat flour lipids. Supercritical fluid extraction (SFE) with carbon dioxide has provided that medium and the method is fully automatic. Crude fats or nonstarch free lipids (FL) were extracte...

Extraction of DNA from mouse tails.

Extraction of rotavirus from human feces by treatment with lithium dodecyl sulfate.

A procedure has been developed for the isolation of rotavirus from human fecal specimens based on the resistance of the virus to treatment with cold 1% lithium dodecyl sulfate at neutral pH. A single detergent treatment of fecal material followed by low- and high-speed centrifugations yielded a virus suspension of sufficient purity for viral ribonucleic acid to be analyzed directly by electroph...

Experiments in DNA extraction and PCR amplification from bighorn sheep feces: the importance of DNA extraction method.

Reliability of genotyping is an issue for studies using non-invasive sources of DNA. We emphasize the importance of refining DNA extraction methods to maximize reliability and efficiency of genotyping for such DNA sources. We present a simple and general method to quantitatively compare genotyping reliability of various DNA extraction techniques and sample materials used. For bighorn sheep (Ovi...